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KMID : 0370220030470050311
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Yakhak Hoeji
2003 Volume.47 No. 5 p.311 ~ p.318
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Inhibitory Effect of Eurya emarginata on the Production of Pro-inflammatory Cytokines in Murine Macrophage RAW264.7
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박수영/Park SY
이혜자/현은아/문지영/양홍철/이남호/김세재/강희경/Lee HJ/Hyun EA/Moon JY/Yang HC/Lee NH/Kim SJ/Kang HK
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Abstract
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Eurya emarginata (Thunb.) Makino (Theaceae) is distributed in coastal areas of island. The leaves of Eurya are used in the traditional medicine of the coastal ar eas of jeju island with the aim of diuresis or to treat ulcers. Nevertheless, there are few reports on the biological activity and constituents of E. emarginata. In this study, we investigated the pharmacological activity of the solvent extracts of E. emarginata on the several inflammatory markers (TNF- a, IL-1β, IL-6, NO, iNOS and COX-2). Also we examined the antioxidizing effect of the solvent extracts by determination of DPPH radical-scavenging activity. Among the solvent fractions, EtOAc and BuOH extracts showed potent radical scavenging activity (RC50=10.9 and 12.7 respectively). The subfractions of EF 5-4-6-3-2 and BF 1 potentially inhibited the mRNA expression of pro-inflammatory cytokins (IL-1β, IL- 6 and TNF-a) at the concentration of 100 μg/ml. Also the fractions inhibited the mRNA expression of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-a) and protein expression of iNOS and COX-2 at the concentration of 100 μg/ml. And then, the inhibition of iNOS was correlated with the decrease of nitrite level. These results suggest that E. emarginata may have anti-inflammatory activity through the inhibition of pro-inflammatory cytokines.
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